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Absolute Eosinophil Count (AEC): Grading Eosinophilia from the CBC Differential

Deriving the absolute eosinophil count from WBC and the eosinophil percentage, the mild/moderate/severe 500-1500-5000 per microliter grading, the hypereosinophilia threshold, a worked example, and interpretation pitfalls.

Run it: Absolute Eosinophil Count (AEC).

What it is

The absolute eosinophil count (AEC) is the actual number of eosinophils per microlitre of blood — a far more reliable figure than the eosinophil percentage alone, because a percentage moves with the total white count. Eosinophilia is graded, and treatment urgency tracked, off the absolute number, not the percent.

Formula

$$ AEC,(/\mu L) = WBC,(\times10^9/L) \times \frac{eos,%}{100} \times 1000 $$

The x1000 converts the WBC from x10⁹/L (thousands per µL) into cells per µL. (Equivalently: WBC in cells/µL x eos%.)

Grading

Hypereosinophilia is defined as >= 1500 /µL on two occasions (>= ~1 month apart) or significant tissue eosinophilia.

When to use

Any time the differential flags eosinophils and you need to know whether it is trivial atopy or a count that demands a work-up. Converting the percentage to an absolute count is the first triage step before chasing causes.

Worked example

WBC 12 x10⁹/L, eosinophils 8%:

960/µL falls in the mild eosinophilia (500–1499/µL) band — consistent with common benign causes; reassess with the clinical picture rather than launching an exhaustive work-up.

Pitfalls

Run it: Absolute Eosinophil Count (AEC)


Decision support for qualified clinicians only — verify against current primary guidelines and your clinical judgement.

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References

Last updated 2026-06-28.

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